Investigations on Malt Amylase. I. Viscometrical Determination of alpha-Amylase.

Bacterial alpha amylase pdf

Of alpha-amylases and other starch-digesting enzymes from bacteria and plants.ess was supplemented with bacterial and fungal amylases or scalded flour. Immunochemical characteristics of alpha-amylase sensitization.Alpha-amylase production and its activity was compared in two different. Bacterial amylases are resistant to high temperatures, they can active in wide pH.fungal amylascs lowest, liqu...

New saccharogenic determination of alpha-amylase in serum and urine.

I describe a new kinetic enzymatic saccharogenic method for assaying alpha-amylase in human serum and urine. alpha-Amylase liberates maltose from starch. This is successively acted on by alpha-glucosidase, mutarotase, and glucose dehydrogenase. The resulting conversion of NAD+ to NADH, measured at 340 nm, during a 20-min incubation reflects amylase activity. Endogenous glucose is destroyed befo...

Purification of alpha amylase pdf

Molecular characterization of the MalT-dependent periplasmic alpha-amylase of Escherichia coli encoded by malS.

malS, the gene encoding the periplasmic alpha-amylase, is under the regulatory control of the MalT protein, the gene activator of the Escherichia coli maltose system. We sequenced the DNA region encoding malS and its control elements. malS consists of an open reading frame of 2,028 base pairs encoding a protein of 676 amino acids with a deduced molecular weight of 75,664 including a typical ami...

KINETICS OF MALT a-AMYLASE ACTION* BY SIGMUND SCHWIMMER

The action of cw-amylase on undegraded starch and on dextrins of high molecular weight is roughly 100 times as rapid as its action on the smaller dextrins formed subsequently. The mechanism of this action has been studied in two ways. By kinetic methods, the course and rate of hydrolysis at varying substrate concentrations are determined. Thus Myrback and Johansson (1) calculated the affinities...